ALLIED DATA TTA128-M2 DRIVER DETAILS:
|File Size:||34.2 MB|
|Supported systems:||ALL Windows 32x/64x|
|Price:||Free* (*Free Registration Required)|
ALLIED DATA TTA128-M2 DRIVER
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After a 20 min incubation, the absorbance of each well and the phosphate standards, which are provided by the kit, Allied Data TTA128-M2 determined by SpectraMax M2 plate reader Molecular Devices, Sunnyvale, CA at nm. After pulse treatment, cells were fixed with 3. The cells were then washed at least once with PBS and permeabilized with 0.
Subsequently, cells were washed twice with PBS and the coverslips were mounted on the slides. Experiments were repeated multiple times and the data reflects pooled results. The automated Allied Data TTA128-M2 determines the average cell colony perimeter and the number of valid cells per well. The cell area data was averaged and normalized to display only the change in area compared to control conditions.
The assessment of cell count was measured as previously described Transfected cells were incubated for an additional 24 h prior to treatment with etoposide, nacodazole, aphidicolin, paclitaxel or BrdU, where indicated. For the BrdU treatment, cells were pulse treated for 1 h with a BrdU analog, whereas all other conditions were treated with drugs for approximately 4 h. Allied Data TTA128-M2 were then stained according to the protocol provided by the manufacturer Millipore, Billerica, MA. Plates were scanned using Cellomics ArrayScan ThermoFisher Scientific and the number of cells was automatically Allied Data TTA128-M2. Each condition had 6 replications and 8 fields per well were analyzed using high content scanning software.
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Representative images are shown reflecting data captured from one of multiple experiments that produced identical results. The data is presented as the average protein synthesis in bar graphs and Allied Data TTA128-M2 images of HeyA8 cells are depicted in the figure.
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