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Shirras, unpublished results where it is assumed to have a role in the metabolism of, as yet unidentified, biologically active peptides involved in neuroendocrine signalling and reproduction.

ACE activity has Acer 35c found in the gonads and accessory glands of several insect species in addition to D. Recently, we have provided evidence for a role for ACE in reproduction in the mosquito, A.

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We have shown that feeding two different ACE inhibitors to male A. In Acer 35c, ACE inhibitors introduced in the blood meal, resulted in a dose-dependent effect on brood-size, but had no effect on oocyte development, nor the rate of digestion of the blood. The only observable difference between inhibitor-fed and control insects was that the inhibitor-fed females could not lay eggs, even 8 days after the blood meal, suggesting that the blood-induced ACE is involved in the control of egg-laying [ 19 ]. To understand the mechanism of ACE induction in blood-fed mosquitoes and the role of the enzyme in mosquito reproductive physiology, we have characterised the ACE gene family in Anopheles gambiae, a mosquito responsible for the Acer 35c of the human malaria parasite and whose genome has recently been sequenced [ 20 ].

The partially annotated A gambiae genome [ 21 ] reveals Acer 35c eight and twelve potential ACE-like genes. We now report the correct number of A. Results ACE-like genes in the A.

BLAST searching of the genome and an examination of the predicted genes, however, shows that not all ACE-like proteins have been predicted, while several of those that have been identified have missing exons, or are hybrids, composed of exons from Acer 35c than one ACE-like gene. In order to confirm the number and organisation of ACE-like genes in the A.


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